Fig 1. OneMinute® prevents the loss of transferred proteins comparing to traditional stripping buffer.

OneMinute: 30sec at RT, washing 3x 10 sec, No re-blocking.                             Competitor: 15min at 50℃, washing 3x 10min, re-blocking 1 hour, washing 3x 15min

5% milk blocked nitrocellulose Western blots of 293 cell lysate were probed with Rabbit-anti-Human JNK1 (Protein1, Santa Cruz, sc-747) at 1:200 dilution, HRP-Goat-anti-Rabbit (Amersham) at 1:10,000 dilution. The signal was detected using ECL substrate (Panel A). Blots were stripped with either 2ml of OneMinute® Stripping Buffer (30 sec, RT) or 10ml of competitor stripping buffer (15min,  50℃) (Panel B). OneMinute® Buffer treated blot was washed 3x 10sec with TBST, and performed re-probing directly without re-blocking. Competitor stripping buffer treated blot was washed 3x 10min, blocked by 5% milk for 1 hour, and washed 3x 15min with TBST. Blots were re-probed with Mouse-anti-Human RIPK1 (Protein2, BD, 610458) at 1:200 dilution, HRP-Goat-anti-Mouse (Amersham) at 1:2,000 dilution and detected with ECL substrate (Panel C).