5% milk blocked Nitrocellulose
Western blots of 293 cell lysate were probed with
Mouse-anti-Human Actin
(Sigma, A-5441) at 1:5000 dilution, HRP-Goat-anti-Mouse
(Amersham) at 1: 2,000 diluiton. The signal was detected using ECL substrate (Panel A). Blots were stripped with either
2ml of OneMinute®
Stripping Buffer ( 1min,
RT), or 10ml of competitor stripping buffer (P's R
product, 15min, RT)
(Panel B). To further test the removal of primary
antibody, the stripped blots were re-probed with identical
secondary antibody and detected with ECL substrate (Panel
C).
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