OneMinute®Advance
Western Blot Stripping Buffer 2nd
generation especially for high-affinity antibodies
GM6031
OneMinute®
Advance WB Stripping
Buffer
25
Tests
$189.00
OneMinute®Advance Western Blot Stripping Buffer has
extraordinary efficiency to strip off high-affinity
antibodies without loss of transferred proteins, which
ensures multiple times re-probing.
Re-blocking
is NOT necessary and the blot is ready for re-probing in 6
min (5 min pretreatment and 1 min stripping). It
represents the world-best Western Blot stripping buffer.
Highlights:
Especially
for high-affinity antibodies
Fast
and easy
No
significant loss of transferred
proteins
Multiple times of re-probes: ~10
times
Re-blocking is usually not necessary
Perform at RT, no offensive odor
Work
for both nitrocellulose and PVDF
membranes
Protocols:
1. Pretreatment of blot with Stripping Enhancing
buffer for 5min
2.
Immerse membrane in OneMinute®
Western Blot Stripping Buffer for 30 sec.
3. Wash the blot with Washing Buffer (TBST or PBST)
3 x 10 seconds*. *The
blot is ready for re-probing. Re-blocking
membrane is usually not necessary.
Fig
8. OneMinute®Advance is more
efficient than Pierce’s Restore and RestorePlus
293 cell lysate was probed with high-affinity
mouse-anti-human actin antibody (left panel). Blots were then stripped either with
OneMinute®Advance Stripping Buffer (5 min pretreatment + 1min stripping) or Pierce’s Restore and Restore Plus (15min stripping). The blots were then
re-probed with mouse-anti-human RIPK1 antibody (Right panel).
Please click the figure for details.
