OneMinute®Plus
Western Blot Stripping Buffer Longer
shelf life, and economy-size available
GM6011
OneMinute®Plus
WB Stripping Buffer
25
Tests
$139.00
GM6012
OneMinute®Plus
WB Stripping
Buffer
4
Tests
$43.00
GM6015
OneMinute®Plus
WB Stripping Buffer
100
Tests
$449.00
OneMinute®Plus
Western Blot Stripping Buffer has a little bit better
stripping efficiency than OneMinute® Western Blot
Stripping Buffer, and much longer shelf life. It provides
economy-size for those customers who prefer to spend less
for a larger volume, but can not use it up in short time.
OneMinute®Plus
Western Blot Stripping Buffer immediately
makes your membrane ready for re-blotting in
ONE minute. It
spends only 30 seconds for stripping and 3x 10
seconds for washing.
Highlights:
Longer
shelf life: ~18
months
a
little bit higher efficiency than OneMinute®
Western Blot Stripping Buffer
Faster, easier and more efficient: only
need 1 min
No
significant loss of transferred
proteins
Multiple times of re-probes: ~10
times
Re-blocking is usually not necessary
Perform at
RT, no offensive odor
Work
for both nitrocellulose and PVDF
membranes
Protocols:
1.
Immerse membrane in OneMinute®Plus
Western Blot Stripping Buffer
for 30 sec.
2. Wash the blot with Washing Buffer
(TBST or PBST)
3 x 10 seconds*. *The
blot is ready for re-probing. Re-blocking
membrane is usually not necessary.
Fig
10. OneMinute®Plus is a bit better than OneMinute® stripping buffer, OneMinute®Advance has the highest stripping efficiency.
293 cell lysate was probed with high-affinity actin antibody (left panel). Blots were then stripped with OneMinute®
Stripping Buffer, or OneMinute®Plus Stripping Buffer, or PBST control (-), or
OneMinute®Advance Stripping Buffer. The blots were then re-probed with 2nd antibody to test the presence of actin antibody(Right panel). Please
click the figure for details.
