DeepCatch™ Cell-Free Total Nucleic Acids Isolation Kit

PRODUCT OVERVIEW

Full-spectrum cfNA recovery from a single plasma or serum sample

Cell-free nucleic acids contain a broad range of DNA and RNA fragment populations, including cfDNA, cfRNA, small RNA, ultra-short cfNA, degraded sub-nucleosomal fragments, and extracellular vesicle-associated RNA.

Conventional cfNA isolation methods often show reduced recovery for fragments below 30 nt, with severe losses below 25 nt (see DeepCatch™ Technology Fig 1). This can distort the native cfNA profile and limit access to low-abundance or ultra-short biomarker classes.

The DeepCatch™ Cell-Free Total Nucleic Acids Isolation Kit is designed to overcome this limitation. It enables high-yield, carrier-free recovery of both cfDNA and cfRNA from plasma or serum, preserving a broader and more complete cell-free nucleic acid landscape for downstream molecular analysis.

KEY BENEFITS

Designed for complete cfNA recovery and advanced liquid biopsy research

• Full-spectrum cfDNA and cfRNA co-isolationRecover cfDNA and cfRNA from the same plasma or serum sample, reducing sample consumption, workflow time, and inter-assay variability.
• Ultra-short cfNA recovery down to 10 nt (see DeepCatch™ Technology Fig 1)Preserve short and ultra-short cfNA fragments that are often lost using conventional extraction methods.
• User-selectable recovery range (see DeepCatch™ Technology Fig 3)Choose recovery down to 15 nt for standard cfNA profiling or down to 10 nt for discovery applications requiring broader fragment coverage.
• Carrier-free performance (see DeepCatch™ Technology Fig 2)No carrier nucleic acid, PEG, glycogen, LPA, or similar precipitation additives are required, reducing artificial background and carryover concerns.
• Scalable sample input volumesStandardized for 1 mL plasma or serum, with flexible protocol guidelines allowing seamless scale-down for limited samples (e.g., 200 µL) or scale-up for larger volumes (up to 3 mL) to maximize low-abundance biomarker yield.
• Downstream application compatibility (see DeepCatch™ Technology Fig 5A, 5B)Purified cfNA is suitable for qPCR, ddPCR, adapter ligation, ligation-based library preparation, and next-generation sequencing.

Recovery Modes

Select the cfNA recovery range that fits your study.

Target Molecules

Capture both DNA and RNA biomarker classes from the same sample. The DeepCatch™ Cell-Free Total Nucleic Acids Isolation Kit supports recovery of a broad range of cell-free nucleic acid species.

Applications

• Liquid biopsy biomarker discoveryRecover DNA- and RNA-based biomarkers from the same plasma or serum sample for broader molecular profiling.
• cfDNA fragmentomicsPreserve short, degraded, and sub-nucleosomal cfDNA fragments for size-distribution, end-motif, and fragmentomic analysis.
• ctDNA signal enrichment researchSupport studies focused on low-abundance tumor-derived fragments and short-fragment populations.
• MCED and MRD researchEnable high-sensitivity recovery of cfNA species relevant to multi-cancer early detection and minimal residual disease research.
• Fetal cfDNA studiesRecover a broad cfDNA fragment spectrum from limited plasma inputs.
• cfRNA and small RNA analysisEnable analysis of miRNAs, isomiRs, tRFs, piRNAs, Y RNA fragments, and EV-associated small RNAs.
• NGS library preparationPrepare clean, carrier-free cfNA input for ligation-based library preparation and sequencing.

Downstream Fractionation Option

Recover the full cfNA landscape first, then fractionate for higher-resolution discovery.

Recover the full cfNA landscape first, then use precision fractionation to focus on the most informative fragments. After cfNA isolation, the eluate can be directly processed with the DeepCatch™ Small Nucleic Acid Fractionation Kit for precise size selection.

This integrated workflow allows researchers to first preserve the full cfNA landscape, then enrich specific fragment populations for more focused downstream analysis.

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