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PathwayScanTM Multi-Pathway Reporter Kit
Making
New Discoveries Has Never Been Easier
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| GM8001
| PathwayScan
Multi-Pathway Reporter Kit |
1
Kit |
Request Quote |
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PathwayScan
is a simple and facile way to simultaneously screen
46 signal
pathways. It dramatically promotes new discoveries. Making new discoveries
has never been easier (Fig1,2,3).
PathwayScan
Multi-Pathway Reporter Kit provides a comprehensive collection of proprietary
luciferase reporters for 46 transcription factors (TFs). It can be easily performed in a single experiment to scan 46 signaling pathways. Each of the TFs was carefully chosen to monitor its corresponding signaling
pathway (see table below).
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PathwayScan works best for:
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Looking for novel signaling pathways in which a gene is involved
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Identifying signaling pathways influenced by a mutant gene,
microRNA, siRNA, shRNA
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Studying crosstalk of signal pathways
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Identifying signaling pathways influenced by various stimuli (cytokines,
chemokines, chemicals, hormones, etc.)
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Signaling pathway profiling
in a variety of scenarios
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Screening signaling pathways influenced by
home-made cells (transient or stable,
overexpressed or knock-down, knock-out or knock-in)
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Identifying phenotype of
transgenic, knock-out or knock-in mice
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Drug screening
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PathwayScan benifits:
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Detect both activation and inhibition of transcription factors
(Fig
1, 2,
3). It is a huge benefit and can be rarely achieved by others.
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Simultaneously screen 46 different signaling
pathways of cancer, immune and stem
cells etc.
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One experiment leads to many new discoveries
(Fig
1, 2,
3)
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The
list of 46 transcription factors and their
corresponding pathways |
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Cytokine EGF is known to activate transcription factors such as Erk, AP-1, STAT3 , EGR-1 and GATA. In one
single experiment of PathwayScan on EGF, the activations of Erk (66-fold), AP-1 (4-fold) , STAT3 (3.5-fold), EGR-1 (9.7-fold) and GATA (3.3-fold) were confirmed
(Fig 1). In addition, 4 transcription factors were found for the first time to be activated or inhibited by EGF (labeled as “New discovery”).
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| Fig
1. EGF stimulated HEK 293 cells were analyzed by
PathwayScan Multi-Pathway Reporter Kit. Please click the figure for details. |
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| Protein
kinases are involved in most, if not all, of
signal transduction pathways. It is known that
IKK2 activates NFkB and plays a critical role in
NFkB signaling pathway. A single test of
PathwayScan on IKK2 not only confirmed its
activation on NFkB (25-fold) and Erk (2.8-fold) (Fig 2), but also revealed for the first time
that 3 transcription factors were remarkably
activated and 2 others were strongly inhibited
(labeled as “New discovery”). |
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| Fig
2. IKK2-CA overexpressed HEK 293 cells were
analyzed by PathwayScan Multi-Pathway Reporter Kit. Please
click the figure for details. |
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| Pathway-specific stimulus is routinely employed if a gene/treatment/maneuver has inhibitory effect on signals. For example, in order to study optineurin
(OPTN)’s inhibition on NFkB, TNF is necessary to boost NFkB signal so that OPTN’s inhibition becomes detectable. In contrast, PathwayScan is so sensitive that the inhibition is detectable even without TNF
stimulation. As shown in Fig 3, NFkB was successfully inhibited for 9-fold by OPTN
(green arrow). Moreover, 4 transcription factors were found to be dramatically inhibited or activated (labeled as “New discovery”). |
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| Fig
3. Optineurin
overexpressed HEK 293 cells were analyzed by
PathwayScan Multi-Pathway Reporter Kit.
Please
click the figure for details. |
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Ordering
Information |
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